Journal: bioRxiv

Article Title: Combinatorial morphogenetic and mechanical cues to mimic bone development for defect repair

doi: 10.1101/561837

Figure Lengend Snippet: ( A ) Representative 3-D microCT defect reconstructions of mid-shaft transverse (top) and sagittal (bottom) sections at week 12, selected based on mean bone volume. Dashed circles show 5 mm defect region. Rectangular boxes illustrate transverse cutting planes. Scale bar, 1 mm. ( B ) Morphometry analysis of bone volume fraction, ( C ) trabecular number, ( D ) trabecular thickness, ( E ) trabecular separation, shown with native femoral head properties (N = 3; dotted lines with gray shading: mean ± SD; † p<0.05 vs. femoral head), and ( F ) ectopic bone formation (i.e., bone extending beyond the 5-mm defect diameter) at week 12 (N = 7-10 per group). Individual data points shown with mean ± SD. Comparisons between groups were evaluated by two-way ANOVA with Tukey’s post-hoc tests. Repeated significance indicator letters (a,b,c) signify p > 0.05, while groups with distinct indicators signify p < 0.05.

Article Snippet: DICOM-exported files were processed for 3-D analysis (CTAn software, Skyscan; Bruker) using a gauss filter at 1.0 pixel radius and a global threshold range of 28-255 for all samples.

Techniques:

Journal: bioRxiv

Article Title: Combinatorial morphogenetic and mechanical cues to mimic bone development for defect repair

doi: 10.1101/561837

Figure Lengend Snippet: ( A ) Longitudinal quantification of bone volume at weeks 4, 8, and 12 by in vivo microCT (N = 4-11 per group). Data shown with mean ± SD. ( B ) Bone volume accumulation rate, defined as bone volume accrual over each 4-week interval. Box plots display median as horizontal line, mean as +, inter-quartile range as boxes, and min/max range as whiskers. ( C ) Representative 3-D microCT reconstructions showing bone formation per group over time. Representative samples were selected based on mean bone volume at week 12. Scale bar, 3 mm. Comparisons between groups were evaluated by two-way repeated measures ANOVA with Tukey’s post-hoc tests. Repeated significance indicator letters (a,b,c) signify p > 0.05, while groups with distinct indicators signify p < 0.05 at each time point. Comparisons between time points were not assessed.

Article Snippet: DICOM-exported files were processed for 3-D analysis (CTAn software, Skyscan; Bruker) using a gauss filter at 1.0 pixel radius and a global threshold range of 28-255 for all samples.

Techniques: In Vivo

Journal: bioRxiv

Article Title: Combinatorial morphogenetic and mechanical cues to mimic bone development for defect repair

doi: 10.1101/561837

Figure Lengend Snippet: ( A ) Representative 3-D microCT reconstructions, with bone formation illustrated at mid-shaft transverse (top) and sagittal (bottom) sections at week 12, selected based on mean bone volume. Dashed circles show 5 mm defect region. Rectangular boxes illustrate transverse cutting planes. Note, due to minimal bone regeneration, additional transverse sections for stiff and compliant no growth factor controls were derived from the proximal end of the defect (small dashed circles and arrows). Scale bar, 1 mm. ( B ) Morphometric analysis of bone volume fraction, ( C ) trabecular number, ( D ) trabecular thickness, and ( E ) trabecular separation at week 12 (N = 4-11 per group), shown with corresponding measured parameters of femoral head trabecular bone (N = 3; dotted lines with gray shading: mean ± SD; † p<0.05 vs. femoral head). Individual data points shown with mean ± SD. Comparisons between groups were evaluated by two-way ANOVA with Tukey’s post-hoc tests. Repeated significance indicator letters (a,b,c) signify p > 0.05, while groups with distinct indicators signify p < 0.05.

Article Snippet: DICOM-exported files were processed for 3-D analysis (CTAn software, Skyscan; Bruker) using a gauss filter at 1.0 pixel radius and a global threshold range of 28-255 for all samples.

Techniques: Derivative Assay

Journal: bioRxiv

Article Title: Combinatorial morphogenetic and mechanical cues to mimic bone development for defect repair

doi: 10.1101/561837

Figure Lengend Snippet: ( A ) Morphometry analysis of proximal vs. distal bone volume distribution (N = 4-11 per group; p<0.05), and ( B ) ectopic bone formation (i.e., bone extending beyond the 5-mm defect diameter) shown with BMP-2 soaked on collagen data (N = 9; dotted line with green shading: mean ± SD). ( C ) Representative 3-D microCT reconstructions of native femoral head transverse (top) and sagittal (bottom) sections, selected based on mean bone volume. Dashed circles show 5 mm defect region. Rectangular box illustrates transverse cutting plane. Scale bar, 1 mm. ( D ) Morphometry analysis of connectivity density, ( E ) degree of anisotropy, and ( F ) structure model index within the defect region (N = 4-11 per group), shown with native femoral head properties (N = 3; dotted lines with gray shading: mean ± SD; † p<0.05 vs. femoral head). Individual data points shown with mean ± SD. Comparisons between groups were evaluated by two-way ANOVA with Tukey’s post-hoc tests. Repeated significance indicator letters (a,b,c) signify p > 0.05, while groups with distinct indicators signify p < 0.05.

Article Snippet: DICOM-exported files were processed for 3-D analysis (CTAn software, Skyscan; Bruker) using a gauss filter at 1.0 pixel radius and a global threshold range of 28-255 for all samples.

Techniques: